Rules for sampling and transmission of samples for testing for the presence of specific antibodies against rabies by the FAVN method.
Blood for testing animals for specific antibodies against rabies must be collected by a veterinarian at least 30 days after vaccination (the time required for the formation of specific antibodies in the body) and 3 months before transporting the animal to the European Union (according to European Commission № 576/2013).
Without identification of the animal (chipping) blood test for the presence of specific antibodies is not performed.
Blood sampling in animals is carried out regardless of food intake (starvation diet does not affect the amount and presence of antibodies in the blood).
In dogs, cats and fur animals, blood is taken from the lateral subcutaneous vein of the forearm or shin.
Blood is collected in an amount of 2.5-5.0 ml from the animal to obtain, after settling or centrifugation, 0.5-1.0 ml of serum for research.
The laboratory takes samples of whole blood, provided it is transported in a thermal container with a cooler. The period of blood supply to the laboratory from the moment of selection should not exceed 3 hours.
If it is not possible to deliver blood to the laboratory within 3 hours of sampling, serum or plasma should be obtained.
Obtaining blood serum by settling is carried out according to the following procedure. After collection, the blood is left in a syringe in an upright position or placed in a test tube and left for 1-2 hours at room temperature, then transferred to a refrigerator at 4-8 0C for 5-10 hours. The color of the removed serum should be from light yellow to pink (slight hemolysis is allowed, but transparency should be maintained). The serum is taken in a test tube type “epindorf” in an amount of at least 0.5 ml.
Obtaining blood plasma by centrifugation is carried out according to the following procedure. The blood is placed in a centrifuge tube and centrifuged at a speed of 2-4 thousand revolutions per minute on a microcentrifuge for 8-10 minutes. Blood plasma is taken in a test tube type “epindorf” in an amount of at least 0.5 ml.
It is allowed to freeze whey samples for storage and transportation (repeated thawing-freezing can affect the results of the study!).
In addition to blood serum, the following should be provided to the laboratory:
– Cover note – filled in Ukrainian and English (in block letters – based on passport data of the owner and the animal).
– Photocopy (all completed pages) of the international passport of the animal (including pages with a mark of identification (chip number) and vaccination).
– Photocopy of the passport of the owner (first page) or the person who will accompany the animal during transportation.
Frequently asked Questions
To take a sample in the laboratory, you must have the following documents:
- foreign passport of the owner (owners) - first page;
- animal veterinary passport To take blood for the presence of antibodies to the rabies virus, feeding the animal immediately before the procedure does not matter.